Doublecortin and doublecortin-like are expressed in overlapping and non-overlapping neuronal cell population: Implications for neurogenesis

Publication Type:

Journal Article

Source:

J Comp Neurol, Volume 13, p.2805–2823 (2012)

ISBN:

1096-9861 (Electronic)00

DOI Name (links to online publication)

10.1002/cne.23144

Abstract:

We have characterized the expression of doublecortin-like (DCL), a microtubule associated protein involved in embryonic neurogenesis that is highly homologous to doublecortin (DCX), in the adult mouse brain. To this end, we developed a DCL-specific antibody and used this to compare DCL expression with DCX. In the neurogenic regions of the adult brain like the subventricular zone (SVZ), the rostral migratory stream (RMS), the olfactory bulb (OB) and the hippocampus, DCL co-localizes with DCX in immature neuronal cell populations. In contrast to DCX, we also found high DCL expression in three other brain regions with suspected neurogenesis or neuronal plasticity. Firstly, the radial glia-like, hypothalamic tanycytes show high DCL expression that partly co-localizes with the neural stem cell marker vimentin. Secondly, DCL expression is found in cells of the suprachiasmatic nucleus (SCN), which lacks expression of the adult neuron marker NeuN. Thirdly, a novel region exhibiting DCL expression is part of the olfactory tubercle where DCL is found in the neuropil of the islands of Calleja (ICj). Our findings define DCL as a novel marker for specific aspects of adult neurogenesis, which partly overlap with DCX. In addition, we propose unique roles for DCL in adult neurogenesis and we suggest high levels of neuronal plasticity in tanycytes, SCN and ICj. J. Comp. Neurol., 2012. (c) 2012 Wiley-Liss, Inc.

18/01/2013