The B cell antigen receptor controls integrin activity through Btk and PLCgamma2

Publication Type:

Journal Article

Source:

J Exp Med, Volume 198, Number 10, p.1539-1550 (2003)

ISBN:

0022-1007 (Print)0022-10

DOI Name (links to online publication)

10.1084/jem.20011866

Keywords:

Agammaglobulinemia/metabolism; Animals; B-Lymphocytes/metabolism; Calcium; Cell Adhesion/physiology; DNA-Binding Proteins/metabolism; Humans; Integrins/*metabolism; Mice; Phosphatidylinositol 3-Kinases/metabolism; Phospholipase C gamma; Protein Kinase C/m

Abstract:

Integrin-mediated adhesion and B cell antigen receptor (BCR) signaling play a critical role in B cell development and function, including antigen-specific B cell differentiation. Here we show that the BCR controls integrin alpha4beta1 (VLA-4)-mediated adhesion of B cells to vascular cell adhesion molecule-1 and fibronectin. Molecular dissection of the underlying signaling mechanism by a combined biochemical, pharmacological, and genetic approach demonstrates that this BCR-controlled integrin-mediated adhesion requires the (consecutive) activation of Lyn, Syk, phosphatidylinositol 3-kinase, Bruton's tyrosine kinase (Btk), phospholipase C (PLC)gamma2, IP3R-mediated Ca2+ release, and PKC. In contrast, activation of mitogen-activated protein kinase kinase (MEK) or extracellular signal-regulated kinase (ERK) is not required, and simultaneous activation of MEK, ERK, and PKB is not sufficient either. Furthermore, Btk is also involved in the control of integrin-mediated adhesion of preB cells. The control of integrin alpha4beta1-mediated B cell adhesion by the BCR involves cytoskeletal reorganization and integrin clustering. These results reveal a novel function for the BCR and Btk, i.e., regulation of integrin alpha4beta1 activity, thereby providing new insights into the control of B cell development and differentiation, as well as into the pathogenesis of the immunodeficiency disease X-linked agammaglobulineamia (XLA).

18/01/2013