Imaging of Neuronal Morphology and Network formation

We made the observation that siRNA mediated knockdown of glucocorticoid receptors in the hippocampus specifically affects newborn neurons in the dentate gyrus of the hippocampus, and the maturation of neuronal networks. Using confocal imaging andhigh resolution 3-D reconstruction of neurons that have been transduced with GFP in vivo, we are currently linking the behavioral effects of GR knockdown with changes in migration of adult-born neurons in the hippocampus, their integration in neuronal networks, and the density and maturation of dendritic spines.